Association Between E6 and E7 Human Papilloma Virus Type 16 Oncogen Mutations and P21 Protein Expression in Cervical Cancer

Cervical cancer is a disease characterized by the growth of abnormal cells in cervical tissue. mostly caused Human Papillomavirus (HPV) types 16 and 18. The role genetic factors development malignancy mediated presence mutation HPV oncogene, especially oncogenes E6 E7. Oncogenic proteins E7 initiate dysregulation cellular proliferation apoptotic mechanisms targeting tumor suppressor proteins, such as p21 protein. purpose this study was to assess association between mutations HPV-HR Type pattern protein expression. This cross-sectional conducted at Obstetrics Gynecology Polyclinic Prof. Dr. I.G.N.G. Ngoerah Hospital, from September 2020 2021. material taken tissue patients then put into preservative solution cooler. DNA isolation performed, PCR performed determine positive negative HPV. amplification genes carried out before sequencing analysis gene out. Then, immunohistochemical staining out, followed data using SPSS for windows version 22.0. There were no significant differences characteristics two groups. with expression cases (p-value 0.22).